Will agitation increase the cell density for commonly used bacterial E.coli growth media?
Yes. Agitation will increase the aeration of the E.coli cell growth. Oxygen is required for high density growth of E.coli cells. Agitation is controlled by the shaking speed of a shaker incubator. Agitation alone can increase the cell density for all commonly used bacterial E.coli growth media. Cell density will be further increased with combination of increased agitation and added Mg2+ in the common media.
