Will my hybridoma stop secreting after prolonged culture in the CELLine?
We have no data that indicates selection of non-secreting clones takes place during culture in the CELLine. Even for low antibody secreting cells, no evidence has been found indicating that selection for non-secreting cells takes place. Importantly, a low secreting hybridoma will not revert to a high secretor when cultured in the CELLine. However, in many cases, a low secretor can be tolerated due to the increase in product concentration achieved with the CELLine. Thus, CELLine is very useful for cells which have very low production since CELLine allows protein density to increase substantially.
