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Will protease inhibitors interfere with the intein-mediated cleavage reaction?

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Will protease inhibitors interfere with the intein-mediated cleavage reaction?

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The presence of PMSF (20 µM) or the protease inhibitor cocktail tablets (Sigma, Roche) in the buffers have had no effect on the efficiency of the cleavage reaction. These protease inhibitors can be added to the column buffer for cell lysis.

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