With an objective of a given magnification, why shouldn one use increasingly higher magnification eyepieces to achieve higher total magnification?
To maintain useful magnification, that is, magnification yielding satisfactory clarity and resolution, one must avoid making the specimen appear bigger but not clearer (“empty” magnification). The general rule of thumb in microscopy is that the total magnification should probably not exceed 750x to 1000x the numerical aperture. For example, with a 40x, N.A. 0.65 objective, the total magnification (The multiple of the eyepiece and the objective magnifications) should be between 480x and 650x.
Related Questions
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