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Can I repeatedly thaw and refreeze cryopreserved hepatocytes if I only need to remove a small aliquot of these products from a vial?

April 26, 2017aliquot cryopreserved hepatocytes products refreeze remove repeatedly small thaw vial

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Can I repeatedly thaw and refreeze cryopreserved hepatocytes if I only need to remove a small aliquot of these products from a vial?

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No. We have found that repeated freezing and thawing causes extremely low recovery of viable hepatocytes. That is why we pack our cryopreserved hepatocytes in a volume that is convenient for most single-use applications.