Can the 0.2 micron Immobilon PSQ membrane be used for Immunodetection in a western blot application?
Yes. This membrane, because it is pure PVDF, will have the same compatibility with immunodetection systems as the 0.1 um system. As an advantage, the 0.2 um membrane is more open which will make it easier to block giving it lower non specific binding properties and make it more accessible to antibody probes. Proteins that were difficult to detect on the 0.1 um membrane may be more accessible on the 0.2 um Immobilon PSQ.
