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Can the SNAP i.d. System be used right after blot transfer?

April 26, 2017blot right snap system transfer Used

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Can the SNAP i.d. System be used right after blot transfer?

2 Answers

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• Yes, as long as the blot is still wet. Q. Is any danger of drying the blot if the vacuum is applied for too long? • No, even if you keep the vacuum running for several minutes the blot will not dry since some liquid always remains within the blot holder. Blocking Q. Can I use non-fat dry milk (NFDM) for blocking in the SNAP i.d. System? • Yes, NFDM is excellent blocking reagent for the SNAP i.d. Detection System. The optimal concentration of NFDM in SNAP i.d. is 0.5%. Q. I only have low-fat dry milk available. Can I use that with the SNAP i.d. System? • Yes, follow the same recommendations that are made for using non-fat dry milk. Q. Can I use NFDM at concentration lower than 0.5%? • Yes, we have found adequate blocking with high signal to noise ratios using concentrations of NFDM as low as 0.05%. See the application note entitled: “Key Steps for Successful Immunodetection Using the SNAP i.d. Protein Detection System” (AN1966EN00). Q. Why I am getting poor liquid flow through the blo

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• Yes, as long as the blot is still wet. Q. Is any danger of drying the blot if the vacuum is applied for too long? • No, even if you keep the vacuum running for several minutes the blot will not dry since some liquid always remains within the blot holder. Blocking Q. Can I use non-fat dry milk (NFDM) for blocking in the SNAP i.d. System? • Yes, NFDM is excellent blocking reagent for the SNAP i.d. Detection System. The optimal concentration of NFDM in SNAP i.d. is 0.5%. Q. I only have low-fat dry milk available. Can I use that with the SNAP i.d. System? • Yes, follow the same recommendations that are made for using non-fat dry milk. Q. Can I use NFDM at concentration lower than 0.5%? • Yes, we have found adequate blocking with high signal to noise ratios using concentrations of NFDM as low as 0.05%. See the application note entitled: “Key Steps for Successful Immunodetection Using the SNAP i.d. Protein Detection System” (AN1966EN00). Q. Why I am getting poor liquid flow through the blo