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Does SYBR® Safe DNA gel stain affect cloning efficiencies?

April 26, 2017affect cloning dna efficiencies gel safe stain sybr®

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Does SYBR® Safe DNA gel stain affect cloning efficiencies?

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We have found a distinct advantage to using SYBR® Safe DNA gel stain and non-UV blue light rather than ethidium bromide and UV when purifying DNA from gels for downstream use. The use of SYBR® Safe DNA gel stain with non-UV blue light emitted by Invitrogen’s Safe Imager™ instrument allows you to purify DNA with virtually no UV-induced nicking or crosslinking, resulting in dramatically increased cloning efficiencies.