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For my DNA concentration determination (A260) the BioPhotometer shows a negative value at 230 nm and as a result no ratio A260/A230. What causes the negative A230 value?

April 26, 2017a260 biophotometer concentration determination dna negative NM shows value

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For my DNA concentration determination (A260) the BioPhotometer shows a negative value at 230 nm and as a result no ratio A260/A230. What causes the negative A230 value?

1 Answer

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Posted

Negative A230 values are usually caused by interference components in inadequately concentrated DNA solutions. The negative value should be rectified when you use a lesser sample dilution for the next measurement. Please note that you should measure at 260 nm for a minimum absorbance of 0.1 in order to obtain exact results.