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How do I sterilize complement for assays requiring sterile product?

April 26, 2017assays complement Product requiring sterile sterilize

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How do I sterilize complement for assays requiring sterile product?

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You can sterile filter your complement using a 0.22 um filter, HOWEVER you must dilute your complement first to your optimal dilution before filtering. Otherwise, you may lose complement activity because of the filtration process. You can also use the Baby Rabbit Complement-Tissue Culture Grade which is sterile.