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I am doing retroviral packaging and infection, will my infected cells express tGFP or tRFP?

April 26, 2017cells doing Express Infected infection packaging retroviral tgfp

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I am doing retroviral packaging and infection, will my infected cells express tGFP or tRFP?

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No. The CMV-tGFP and CMV-tRFP is outside of the region that gets packaged by retrovirus. Thus, you cannot use tGFP or tRFP expression to monitor transduction but you can use puromycin selection to generate stable cell lines.