I would like to know the design of your microarray compared with the Affymetrix genechip. Is it compatible to analyze with the Affymetrix program?
The DGRC microarrays are “spotted arrays”. They differ significantly from Affymetrix GeneChips. The DGRC microarrays are fabricated using a robot to print PCR amplified DNA fragments (100-600 bp) to specialized glass microscope slides. They are generally hybridized simultaneously with two or more cDNA samples each labeled with a distinct fluorescent dye. The hybridizations are performed under cover slips and do not require specialized instruments. The microarrays are scanned using microarray scanners used for any spotted array. Affymetrix GeneChips are fabricated by synthesizing short oligonucleotides directly on glass wafers by photolithography. The RNA target is labeled with biotin and following hybridization is stained with streptavadin phycoerythrin conjugate. Consequently each GeneChip can only be hybridized with a single labeled target. The Affymetrix GeneChips are packaged in plastic cartridges that require the use of specialized Affymetrix instruments, a microfluidics instrumen
