Im interested in studying gene expression in my samples. Which Affymetrix arrays should I be considering?
Expression arrays are available for the majority of model organisms. The Affymetrix 3′ IVT expression arrays have become a well-established and trusted method in gene expression profiling. Some of the most popular 3′ IVT arrays are: • Human Genome U133 Plus 2.0 • GeneChip Mouse Genome 430 2.0 • GeneChip Rat Genome 230 2.0 • GeneChip Drosophila Genome 2.0 More recent was the release of the whole-transcript expression arrays. Aimed at being a cheaper alternative to the traditional 3′ IVT arrays, these are of a smaller format and contain only known, well-annotated genes. The figure below illustrates the differences in probe placement between the two array types.
