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Once a stable cell line expressing with pNEBR-R1 is established, do I need to continue culturing the cell line with G418?

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Once a stable cell line expressing with pNEBR-R1 is established, do I need to continue culturing the cell line with G418?

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The stability of each cell line is different therefore, we recommend culturing cells in the presence of the appropriate concentration of G418.

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