What are possible causes of No Signal/Poor Signal in my Western Blot?
• Transfer efficiency may have been poor. Check protein transfer by staining the gel and/or membrane. • Incorrect storage of antibodies or ECL western blotting detection reagents. • Insufficient protein may have been loaded on the gel. Depending on the location of the target protein, membrane or nuclear preparations may be required (instead of whole cell lysates). • Film exposure time may have been too short.