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What is a hybridoma and what are the benefits of monoclonal antibodies?

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What is a hybridoma and what are the benefits of monoclonal antibodies?

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Hybridomas are immortal cell hybrids that secrete epitope-specific monoclonal antibodies. They are produced by the fusion of spleen cells from an immunized mouse with a continuous myeloma cell line. Monoclonal antibodies can be used in a variety of assays, including but not limited to, ELISA, western blot analysis, immunoprecipitation, immunolocalization, flow cytometry, and immunoaffinity chromatography. Monoclonal antibodies can be produced in large quantities for indefinite periods. Polyclonal antibodies recognize a number of epitopes which may be advantageous in some applications; however a mixture of monoclonal antibodies can accomplish the same goals.

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