Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

What is net weight and peptide content?

content NET peptide weight
0
Posted

What is net weight and peptide content?

0

Peptides are normally delivered as fluffy lyophilized material. Due to the nature of the peptides, they may still contain traces of moisture and counter ions on protonated amino functions (N-terminus, Arg, His, Lys, etc.). This is not considered an impurity, but reduces the actual peptide content by approximately 10 to 30%. The net peptide weight is the actual weight of the peptide minus the additional weight from residual moisture and counter ions. In order to ensure accurate peptide concentrations, the non-peptide weight needs to be considered and substracted from the gross peptide weight. An accurate determination of the peptide net weight can be performed by quantitative amino acid analysis, which is also available from Peptide 2.0 upon request. • How do I interpret the data of peptide purity? With exception of crude peptides requested by customers, which will only be confirmed by MS, all synthetic peptides from Peptide 2.0 will be analyzed by HPLC and MS. HPLC profile will show a

Related Questions

What is your question?

*Sadly, we had to bring back ads too. Hopefully more targeted.