What is the threshold cycle or Ct value?
The Ct or threshold cycle value is the cycle number at which the fluorescence generated within a reaction crosses the fluorescence threshold, a fluorescent signal significantly above the background fluorescence. At the threshold cycle, a detectable amount of amplicon product has been generated during the early exponential phase of the reaction. The threshold cycle is inversely proportional to the original relative expression level of the gene of interest.
Related Questions
- The site is running a blended campaign and the threshold value is reached which tells Dialer to stop sending predictive calls. What happens to the calls stacked up in the Dialer queue/MSMQ queue?
- Is there a size threshold for protection of a site of unique value for construction of reservoirs?
- What is the threshold cycle or Ct value?
