Why can the serum insulin level be assayed by solid-phase RIA or the like?
The implants contain bovine, porcine, or rDNA human insulin which produces anti-insulin antibodies in the rodent. The solid-phase RIA or the like is a replacement assay, i.e., radio-active iodine labeled insulin is first bound to the anti-insulin antibodies coated on a solid surface. When the bound iodine-labeled insulin is displaced by serum insulin, the difference in the cpm gives the serum insulin level. If there are anti-insulin antibodies in the serum sample, it will also bind the bound iodine-labeled insulin. Therefore, there are 2 processes of removing the bound iodine-labeled insulin from the solid surface giving results up to 60× higher than expected.
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- Why can the serum insulin level be assayed by solid-phase RIA or the like?
