Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Is it possible to remove the CMV promoter or GFP cassette from the shuttle vectors?

April 26, 2017cassette CMV gfp possible promoter remove Shuttle vectors

0

Posted

Is it possible to remove the CMV promoter or GFP cassette from the shuttle vectors?

1 Answer

0

Posted

It is very difficult because these cassettes were cloned by blunt-ending ligations to ensure more cloning sites in the polylinker. However, you should be able to construct your desired vector by using the pShuttle vector.