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What is the transfection efficiency of jetPEI™-macrophage in monocyte-derived human macrophages?

April 26, 2017efficiency Human macrophages monocyte-derived transfection

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What is the transfection efficiency of jetPEI™-macrophage in monocyte-derived human macrophages?

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The transfection efficiency of jetPEI™-macrophage on human macrophage is approximately 30% according to the jetPEI™-Macrophage protocol or 107 RLU/mg protein. It is essential to grow the human primary macrophage derived from blood monocytes for 7 to 10 days with 100 U/ml of GM-CSF prior to transfection in order for the cells to express functional mannose receptors (C. Furman et al., (2004) Free Radic Biol Med 37: 71-85).