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transfection

Question tag: transfection 71 Questions

0 Votes 1 Ans

Can I use fluorescence microscopy to assess the transfection efficiency of the SureSilencing™ shRNA plasmids with GFP in my model cell line of interest?

assess efficiency fluorescence gfp microscopy model plasmids shrna suresilencing™ transfection

0 Votes 1 Ans

What transfection method should I use with the SureSilencing™ shRNA plasmids?

method plasmids shrna suresilencing™ transfection

0 Votes 1 Ans

Can I use fluorescence microscopy to assess shRNA plasmid transfection efficiency in my model cell line of interest, when using a vector expressing a GFP reporter gene?

assess CELL efficiency fluorescence line microscopy model plasmid shrna transfection

0 Votes 1 Ans

How can I use the SureSilencing™ shRNA plasmids for stable transfection and knock down in a cell line that is already resistant to both neomycin and puromycin?

CELL knock line neomycin plasmids resistant shrna STABLE suresilencing™ transfection

0 Votes 1 Ans

What is the most reliable transfection reagent for delivering shRNA plasmids and siRNA to cells in culture?

cells culture plasmids Reagent shrna siRNA transfection

0 Votes 1 Ans

How does stable transfection of an shRNA-encoding plasmid work?

plasmid STABLE transfection

0 Votes 1 Ans

How long should I wait after transfection before enriching my transfected cells, validating knock down, and looking for phenotypic changes?

cells changes enriching knock phenotypic transfected transfection validating wait

0 Votes 1 Ans

Is it acceptable to correct for transfection efficiency, when determining the level of gene expression knock down by an shRNA expression vector?

acceptable determining efficiency expression gene knock level shrna transfection

0 Votes 1 Ans

Has jetPEI™ been already tested for transfection into plant cells?

cells jetpei™ plant tested transfection

0 Votes 1 Ans

What is the transfection efficiency of jetPEI™-macrophage in monocyte-derived human macrophages?

efficiency Human macrophages monocyte-derived transfection

0 Votes 1 Ans

Is there a plasmid size restriction for DNA transfection using jetPEI™?

dna jetpei™ plasmid size transfection

0 Votes 1 Ans

Can I use antibiotics during transfection with jetPEI™?

antibiotics jetpei™ transfection

0 Votes 1 Ans

How is the plasmid delivered from the cytoplasm to the nucleus when transfection is performed with jetPEI™?

cytoplasm delivered jetpei™ nucleus Performed plasmid transfection

0 Votes 1 Ans

Are the GenePORTER reagents suitable for stable transfection?

geneporter reagents STABLE suitable transfection

0 Votes 1 Ans

What are the GenePORTER Transfection Reagents?

geneporter reagents transfection

0 Votes 1 Ans

How is one transfection reaction defined for the GenePORTER reagents?

defined geneporter REACTION reagents transfection

0 Votes 1 Ans

How do the GenePORTER Transfection Reagents work?

geneporter reagents transfection

0 Votes 1 Ans

Could I use the BLOCK-iT™ Fluorescent Oligo to optimize plasmid transfection?

block-it™ fluorescent oligo optimize plasmid transfection

0 Votes 1 Ans

Can I use the gWiz plasmids for stable transfection?

gwiz plasmids STABLE transfection

0 Votes 1 Ans

Can I use BoosterExpress with my own transfection reagent?

Reagent transfection

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