What is the most reliable transfection reagent for delivering shRNA plasmids and siRNA to cells in culture?
Numerous commercial transfection reagents are available for transfecting cell lines with plasmids or siRNA. Most of these reagents have been engineered for the delivery of either plasmids or siRNA, but not for both. We recommend using SABiosciences’ SureFECT Transfection Reagent (SA-01). SureFECT is incredibly versatile. Its used in a reverse transfection protocol that yields exceptionally high transfection efficiencies in most cell lines, for both plasmids and siRNA molecules. A complete description of this reagent can be found at the following web address: http://www.sabiosciences.com/reversetransfection.
