Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Are any modifications required of routine disk diffusion and MIC methods when testing vancomycin and staphylococci?

April 26, 2017Diffusion disk methods mic modifications routine staphylococci Testing vancomycin

0

Posted

Are any modifications required of routine disk diffusion and MIC methods when testing vancomycin and staphylococci?

1 Answer

0

Posted

Testing should incorporate the following CLSI recommendations: Inoculum: Use direct colony suspension Incubation: 35°C, ambient air, for a full 24 hr. Endpoint: Examine very closely for any indication of growth According to the newest CLSI (formerly NCCLS) standards, a vancomycin-intermediate or –resistant result for staphylococci isolate should be verified by repeating a validated MIC method and the organism identification.