Are the Streptavidin-Coupled Dynabeads® reusable?
• For most applications involving dissociation of biotinylated molecules from streptavidin it is not possible to reuse the beads. The streptavidin-biotin bond is one of the strongest biological bonds known, and the conditions necessary to break this bond also destroy the streptavidin. However, if the Dynabeads Streptavidin have been used in applications such as isolation of DNA binding proteins and release of proteins from DNA is done by gentle methods like using high salt buffers that do not interfere with biotin-streptavidin interaction, the beads with immobilized probe may be reused. I am using Dynabeads® M-280 Streptavidin to isolate DNA binding proteins and added my biotinylated oligo to my protein sample first. I am afraid the B&W buffer suggested for coating of oligos to the beads could break my DNA-protein interaction? • This buffer is intended for immobilizing oligo on to the beads in the absence of protein or when the direct method is applied. For the indirect method, when th
