Can agarose gels be cast with SYBR® Safe DNA gel stain in them?
Yes. Simply substitute a SYBR® Safe DNA gel stain solution for the buffer when preparing the molten agarose. If using the 10,000X SYBR® Safe DNA gel stain concentrate, dilute the concentrated stain 1:10,000 in agarose gel buffer (e.g., 1X TBE or 1X TAE) and add the buffer/stain solution to the powdered agarose. The agarose/SYBR® Safe DNA gel stain mixture may be heated briefly in the microwave.
