Can anyonw explain restriction mapping?
It’s really very simple. So each restriction enzyme cuts at a specific site (a specific DNA sequence usually about 6 nucleotides long). Most enzymes are very specific to that particular sequence and will always cut the same way (either a sticky or blunt end). So say you have a plasmid (circular DNA) and you cut it with Eco RI and Hind III. You simply need to find on the map where those two sites are and with that information you can predict what the cut DNA should look like. EDIT. So your “map” is just a big long list of nucleotides. Each restriction enzyme cuts a very specific nucleotide sequence. So you have to find on the map where those sequences are. Then you simply figure out how much space is between the two sites. That link I listed should help you understand it better. EDIT…. Okay I see now. I can’t really do this for you on here but you should be able to figure it out for yourself. Get some paper and draw a big circle. That’s your plasmid and you know it’s 7950 total. You a
