Can I create cells stably expressing a particular siRNA with the pFIV-copGFP Vectors?
Yes, the pFIV-copGFP Vectors contain all the genetic elements responsible for packaging, transduction, stable integration of the viral expression construct into genomic DNA, and expression of the siRNA effector sequence. The pFIV-PACK Lentiviral Vector Packaging Kit (LV100A-1) provides all the proteins essential for transcription and packaging of an RNA copy of the expression construct into recombinant viral particles. When the construct is expressed in the packaging cell line (HEK 293T cells that stably express the SV40 large T antigen), the pFIV transcripts are efficiently packaged into pseudoviral particles. Following transduction into target cells, the expression cassette is reverse transcribed and integrated into the genome of the target cell. Cells stably expressing the siRNA construct can be selected by FACS.
Related Questions
- Which strand of DNA is packaged when cells carrying the pMAL vectors are superinfected with an M13 helper phage such as M13KO7 (NEB cat #315)?
- Can I create cells stably expressing a particular siRNA with the pFIV-copGFP Vectors?
- What is the risk of using a leukaemia patients own blood cells to create stem cells?
