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Can I fix my cells after staining with eFluorTM Nanocrystal conjugated antibodies?

April 26, 2017antibodies cells conjugated efluortm Fix nanocrystal staining

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Can I fix my cells after staining with eFluorTM Nanocrystal conjugated antibodies?

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Paraformaldehyde (PFA) fixatives have been found to be compatible with eFluorTM Nanocrystal conjugated antibody staining. We have determined that short incubations (30 min or less) of stained cells in 1 or 2% PFA in PBS causes only a minor decrease of the fluorescence signal. Therefore, we recommend a 30 minute fixation followed by a wash step. When optimal staining sensitivity is required, we do not recommend fixing samples.