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Can I fix tissue in formalin and use paraffin embedded sections with the EF5 antibody system?

April 26, 2017Antibody ef5 embedded Fix formalin paraffin system tissue

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Can I fix tissue in formalin and use paraffin embedded sections with the EF5 antibody system?

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Yes but the antibody detection system is only validated for frozen tissue samples. Use no more than 24 hr in fresh formalin, with standard antigen retrieval methods. Expect more background and less signal (so reduced contrast).