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Can I immobilize IgY on Protein A, Protein G or Protein A/G to immunoprecipitate or affinity purify the antigen?

April 26, 2017affinity antigen igy immobilize protein purify

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Can I immobilize IgY on Protein A, Protein G or Protein A/G to immunoprecipitate or affinity purify the antigen?

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No. IgY does not bind to Protein A, Protein G or Protein A/G; however, AminoLink Plus Resin (#20501) or Kit (#44894) can immobilize IgY. The AminoLink Plus Resin is aldehyde-activated and reacts with primary amines on the IgY and forms a covalent linkage.