CAN I STRIP THE HYBRIDIZED TARGET NUCLEIC ACIDS AND RE-USE CUSTOMARRAY CHIPS?
A single CustomArray™ microarray can be stripped three times using the CombiMatrix CustomArray™ Stripping Kit. The CustomArray™ Stripping protocol is based on chemical denaturation of DNA:DNA and DNA:RNA hybrids between oligonucleotide probes on microarrays and corresponding labeled targets. As a result of this denaturation, labeled targers are removed from oligonucleotide probes and washed off microarrays. The resultant stripped CustomArray™ microarrays can be used in the same way as newly synthesized ones. In total, the CombiMatrix Stripping Kit would enable you to use a single CustomArray™ microarray four times.
Related Questions
- CAN I USE BOTH SINGLE-COLOR AND DUAL-COLOR HYBRIDIZATION APPROACHES? HOW DO I LABEL NUCLEIC ACID TARGETS FOR HYBRIDIZATION WITH CUSTOMARRAY CHIPS?
- CAN I STRIP THE HYBRIDIZED TARGET NUCLEIC ACIDS AND RE-USE CustomArray MICROARRAYS?
- CAN I STRIP THE HYBRIDIZED TARGET NUCLEIC ACIDS AND RE-USE CUSTOMARRAY CHIPS?