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Can I use Oligo (dT)16 for my RT reactions in conjunction with the TaqMan® Human Endogenous control Plate (P/N 4309199)?

April 26, 2017conjunction CONTROL DT endogenous Human oligo reactions RT

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Can I use Oligo (dT)16 for my RT reactions in conjunction with the TaqMan® Human Endogenous control Plate (P/N 4309199)?

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No. The reverse transcription of total RNA to cDNA must be done with random hexamers. This is because the 18S rRNA assay cannot evaluate poly A+ purified RNA samples because most of the ribosomal RNA has been removed. For more information, please refer to the protocol.