Can the chitin beads be regenerated?
The chitin beads can be regenerated at least 5 times. After stripping the chitin column with 3 volumes of 0.3 M NaOH (Stripping solution). Allow the beads to soak for 30 minutes, wash the resin with additional 7 column volumes of 0.3 M NaOH. Then wash the resin with 20 bed volumes of water followed by 5 column volumes of Column Buffer. For long term storage, add 0.02% sodium azide to the Column Buffer. The beads should be stored at 4°C. Temporary storage at -20°C will not affect the binding capacity.
