Can the DYEnamic ET Terminator premix be diluted?
Yes. For the DYEnamic ET Terminator sequencing products, the most consistent results are obtained using 8 l of sequencing reagent premix in a reaction volume of 20 l, as described in the sequencing kit protocol book. These are the recommended conditions for routine use and should be the starting point for any optimization that the customer chooses to perform. Some customers may choose to sacrifice some consistency in data to achieve greater economy. If this decision is made, DYEnamic ET Terminator Dilution Buffer (product code US84002) will provide the appropriate buffer conditions, salts, metal cofactors, and stabilizing agents necessary for optimal enzyme activity. In any case, the level of dilution that produces acceptable data must be determined empirically by the customer. Please note that Amersham Biosciences makes every effort to develop products that are consistent in performance over a broad range of conditions. We do NOT recommend or support dilution of our products, and we m
Related Questions
- Im a new customer who has never used DYEnamic ET Terminator Kit on the ABI Prism instrument before. What are the products that Ill need to order for the first time set up before testing the kit?
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