Important Notice: Our web hosting provider recently started charging us for additional visits, which was unexpected. In response, we're seeking donations. Depending on the situation, we may explore different monetization options for our Community and Expert Contributors. It's crucial to provide more returns for their expertise and offer more Expert Validated Answers or AI Validated Answers. Learn more about our hosting issue here.

Can the Streptavidin-Coupled Dynabeads® be used directly in a PCR reaction?

April 26, 2017directly dynabeads® PCR REACTION streptavidin-coupled Used

0

Posted

Can the Streptavidin-Coupled Dynabeads® be used directly in a PCR reaction?

1 Answer

0

Posted

• Yes, the beads can be used directly in PCR, as they do not inhibit enzymatic reaction. Slight PCR-inhibition has been seen when 75 -100 µg of Dynabeads® M-280 Streptavidin was used in 50 µl PCR reaction, and a concentration over 100 µg per 50 µl reaction seems to inhibit PCR completely. Neither Dynabeads® M-270 Streptavidin nor Dynabeads® MyOne™ Streptavidin C1 show any inhibitory effect on PCR at these concentrations.