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Do beacons undergo reverse complement (ie. target) verification and is a close to open ratio analysis performed?

April 26, 2017analysis Beacons complement ratio reverse target undergo verification

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Do beacons undergo reverse complement (ie. target) verification and is a close to open ratio analysis performed?

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Gene Link currently does not perform these analyses as it considers “after the fact”. These analyses do NOT constitute quality of oligo synthesis but rather of design of oligonucleotide. Customers designing their probes should keep the parameters in mind. Gene Link can guide customers to the design of the stem and the loop and the Tm.