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Do the plates need to be equilibrated at room temperatures before reading?

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Do the plates need to be equilibrated at room temperatures before reading?

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Plate temperature could affect the fluorescence reading. Therefore, you need to be consistent with all plates either cool them all down before reading or dont.

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Plate temperature could affect the fluorescence reading slightly. Therefore, you need to be consistent with all plates – either cool them all down before reading or don’t.

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