Do the StrepMAB antibodies also work in immunoprecipitation and pull down assays?
Yes, several applications are possible. We recommend to use the One-STrEP-tag (Strep-tag II is also possible) for the bait protein and to precipitate with Strep-Tactin Superflow or Strep-Tactin magnetic beads using biotin for the elution step. Alternatively, StrepMAB-Classic MacroPrep can be used for precipitation using the Strep-tag peptide for the elution step. Please note, if you have to use classical immunoprecipitation without an elution step, we recommend StrepMAB-Immo MacroPrep for immunoprecipitation because of its nearly irreversible binding to the One-StrEP-tag or Strep-tag II. • Can a tagged protein be detected in situ? Yes. It can be detected via EM or FM with streptavidin which is labelled with colloidal gold or FITC. • Has the Strep-tag® technology been tested in Biacore analysis? Yes. StrepMAB-Immo, high-affinity Strep-tag® II specific monoclonal antibody [Cat.-No.: 2-1517-001], is optimal for capturing SerAla-Strep-tag® II fusion proteins on solid phases such as Biacore
