Does it matter what my final conjugation volume and antibody concentration is once I add the antibody to the eFluor® Nanocrystal solution?
The eFluor® Nanocrystal Conjugation Kits are optimized for a total conjugation volume of 300 µL. However, you may achieve slightly better results if the conjugation volume is kept at approximately 100 µL total conjugation volume. Conjugation volumes as high as 550 µL have been tested, showing that the larger conjugation volume may result in a slight reduction in performance of the final conjugate.
