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Does the protoplasting procedure damage surface components such as plasma membrane receptors?

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Does the protoplasting procedure damage surface components such as plasma membrane receptors?

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A6. Our recent studies of the cytokinin receptors CRE1/AHK4/WOL and the flg22 peptide receptor FLS2, a LRR receptor kinase, demonstrate that the receptors on the surface of protoplasts are intact and can actively perceive specific extracellular signals to trigger intracellular signaling cascades.

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