How are genes counted in DAVID Chart Report?
In DAVID 2.x, all charting tools count the number of unique DAVID gene Ids corresponding to user input gene list user. This means that if two or more of your identifiers represent alternatively spliced forms of the same gene it will only be counted once and reflected in the histograms. This counting method is different from DAVID 1.x in which the user’s gene identifiers are counted. 9. Why are there different levels for GO Annotation? The structured vocabulary created by the Gene Ontology Consortium is a pseudo-hierarchy, or directed acyclic graph (DAG). The different levels provided by GoCharts allows users to annotate lists of genes at different levels within the DAG. Level 1 represents the most general categories and provides the most coverage, whereas Level 5 provides more specific information and less coverage. Users may also annotate their gene lists with all annotations available at all levels, for some genes there will be more than 5. Additionally, users can choose to use only
