How can additional bands from biotinylated proteins on Western blots be avoided?
For blocking biotinylated proteins in Western blots, we offer a Biotin Blocking Buffer containing avidin [Cat.-no.: 2-0501-002]. Use a dilution of 1:1000 in standard Western blot blocking reagent prior to detecting Strep-tag proteins with Strep-Tactin conjugates. Alternatively, monoclonal antibodies against Strep-tag [Cat.-no.: 2-1507-001, 2-1509-001] can be used, which do not recognize biotin. • How can desthiobiotin be separated from the Strep-tag® protein after elution? Either use gel filtration or dialyse the elution fractions against your buffer of choice. Usually this is not necessary, though.
