How can I determine the absorbance or fluorescence for the fully reduced form of alamarBlue®?
The reduced form of alamarBlue® is very unstable in water. For this reason, it is difficult to recommend a standard test for the reduced form. However, the reduced form is very stable in media. To determine the absorbance/fluorescence to be expected from the reduced form for a particular experiment, it is suggested that 1x alamarBlue® be made up in the media intended for use in an autoclavable container. Reduce this preparation by autoclaving for 15 minutes. Remove from the autoclave and allow to cool to room temperature. Swirl the solution several times before taking the appropriate measurements.
