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How can I determine whether amplification occurs from mRNA-derived cDNA or from genomic DNA contamination?

April 26, 2017amplification cDNA contamination determine dna genomic occurs

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How can I determine whether amplification occurs from mRNA-derived cDNA or from genomic DNA contamination?

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The most rigorous method to detect genomic DNA contamination, particularly with the RT² PCR Primer Assays, is to perform a No Reverse Transcriptase (NRT) control. The PCR will have no cDNA template derived from mRNA, and any detectable product could only have been derived from genomic DNA contamination.