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How critical are the fixing and staining times in the protocol?

April 26, 2017critical fixing Protocol staining times

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How critical are the fixing and staining times in the protocol?

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The protocol is quite flexible with regard to fixing and staining times. After gels are fixed or at least 30 minutes, they can be stored in water until the next day. The gel-staining time can range from 5 minutes to 24 hours without incurring additional background.