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How do I elute my target from biotinylated antibody without eluting the antibody off the Dynabeads®?

April 26, 2017Antibody biotinylated dynabeads® elute eluting target

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How do I elute my target from biotinylated antibody without eluting the antibody off the Dynabeads®?

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• You need to use mild elution methods like buffer with high salt (>1 M salt) or low pH. Low pH elution buffers such as 0.1 M glycine•HCl, pH 2.5-3.0 are effective for most antibody-antigen interactions. Note that boiling in SDS will also elute the antibody.