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How do I know whether a PhosphorImager system measurement from a very weak band on my image is statistically valid?

April 26, 2017band image measurement phosphorimager statistically system valid weak

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How do I know whether a PhosphorImager system measurement from a very weak band on my image is statistically valid?

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A. The linear dynamic range of PhophorImager systems begins at one PhosphorImager Count. Given the sensitivity of the PhosphorImager system, if the average pixel value for your band is below one PhosphorImager Count, there are insufficient particle emissions from the band for statistically valid quantitation anyway.