How do I properly prepare space for use with RNA extractions?
When extracting RNA, the work space must be properly treated to eliminate the ubiquitous RNases. It is recommended to wipe down the area with a surface treatment agent such as RNase Zap (Life Technologies) or RNA Away (Molecular BioProducts). Whenever possible, perform all work in a PCR workstation. All plastics, glass, and consumables used in the process should be purchased as RNase-free or treated with RNase Zap, rinsed with Nuclease-free water, and autoclave or baked for 2hr. All reagents purchased should be RNase-free or made fresh with RNase free or DEP-C treated water.
