How does automatic warping adapt if you have a large number of gels in a sample and they differ more down the tree?
In general the automatic warping system is so successful that, provided the gels all belong to the same experiment and have a reasonable level of common pattern, you will have few problems. However, all warping is determined between a ‘reference’ gel and the others. It may be appropriate to select this reference carefully so that it shares the maximum amount of information with the other gels. Alternatively, in these rare cases it may be better to edit the warp manually.